Cell & Tissue Experimental Microscopy

By combining top-rated microscopes with experimental devices we are able to uncover unique information on structural and mechanical properties of human cells. Such obtained knowledge can significantly contribute to a development of novel approaches in artificial tissue engineering, regenerative medicine or in combating cancer cells.

Detail

Our research team deals primarily with the utilization of experimental imaging methods, in particular, fluorescence, holographic and confocal fluorescence microscopy methods in cell and tissue engineering. The team works with cultivation and genetic modification of cells methods, analysis of cell migration, evaluation of metabolism and internal microstructure of cells. Our researchers focus on image processing and analysis of recorded microscopic data. They record and evaluate fast fluorescence processes, volume data or data obtained in long-term experiments. Other activities compose of cardiac and neuronal electrophysiology or electrophysiology of cell clusters and tissues. In terms of our research we deal with the following five topics:

Using advanced microscopy techniques in cell migration analysis

We use advanced microscopy techniques in order to develop recording and analysing methods in migrating cells. Our research includes setting up long-term experiments in controlled incubation conditions that simulate hypoxic conditions. This step is followed by sequential acquisition of volume data, data image processing and a performance of detailed analyses. In order to study acute myocardial infarction and scar tissue scarring process the cell migration mechanisms are utilized.

Research of electrical and mechanical activity in excitable cells

We specialize in linking optical measurement techniques with contact measurement techniques by using the patch clamp technique or next generation microelectrode arrays. The devices are utilized in studies of the electrical and mechanical activity in excitable cells and cell clusters. We have successfully implemented simultaneous measurement methods in calcium dynamics evaluation and the spread of action potential and contraction in cardiac cells.

Studying cell communication in neural networks and cardiac tissue

Our team studies cell communications in neural networks and cardiac tissue by using rhodopsin family and improved genetically encoded voltage sensors. In order to carry out such studies we utilize advanced microscopic techniques, high-speed, extremely sensitive camera devices and optogenetic photo-manipulation device.

Tissue engineering in regenerative medicine

In the field of regenerative medicine the mid-infrared optical coherence tomography (IR OCT) is used for preclinical testing and imaging of in-vivo tissue histological characterization. The objective is to obtain an image on the morphology and the tissue layer regularity in order to determine the scar tissue maturation, quantify the growth of subcutaneous capillaries with the effect of pro-angiogenic therapy or also to quantify the implanted cell or liposome capture for the purpose of transplantation or implantation of muscle tissue or skin.

Characterization of cancer cells by quantitative phase microscopy

The research team focuses on studying methods that are based on simultaneous quantitative phase imaging and mechanical stimulation of living cells in order to evaluate their biomechanical properties. The proposed methods allow for a quantitative cell imaging in dynamic studies of cell morphology and stiffness that are used in oncological research.

 

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